| First Author | Balasubramanian R | Year | 2014 |
| Journal | Genesis | Volume | 52 |
| Issue | 9 | Pages | 827-832 |
| PubMed ID | 25112520 | Mgi Jnum | J:214080 |
| Mgi Id | MGI:5588041 | Doi | 10.1002/dvg.22805 |
| Citation | Balasubramanian R, et al. (2014) Generation and characterization of Lhx9-GFPCreER(T2) knock-in mouse line. Genesis 52(9):827-32 |
| abstractText | LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreER(T) (2) (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9(GCE) (/+) mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9(GCE) (/) (GCE) mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments. genesis 52:827-832, 2014. (c) 2014 Wiley Periodicals, Inc. |
