| First Author | Hou Y | Year | 2021 |
| Journal | Cell Rep | Volume | 34 |
| Issue | 6 | Pages | 108712 |
| PubMed ID | 33567285 | Mgi Jnum | J:306316 |
| Mgi Id | MGI:6714809 | Doi | 10.1016/j.celrep.2021.108712 |
| Citation | Hou Y, et al. (2021) Topographical organization of mammillary neurogenesis and efferent projections in the mouse brain. Cell Rep 34(6):108712 |
| abstractText | The mammillary body is a hypothalamic nucleus that has important functions in memory and spatial navigation, but its developmental principles remain not well understood. Here, we identify progenitor-specific Fezf2 expression in the developing mammillary body and develop an intersectional fate-mapping approach to demonstrate that Fezf2(+) mammillary progenitors generate mammillary neurons in a rostral-dorsal-lateral to caudal-ventral-medial fashion. Axonal tracing from different temporal cohorts of labeled mammillary neurons reveal their topographical organization. Unsupervised hierarchical clustering based on intrinsic properties further identify two distinct neuronal clusters independent of birthdates in the medial nuclei. In addition, we generate Fezf2 knockout mice and observe the smaller mammillary body with largely normal anatomy and mildly affected cellular electrophysiology, in contrast to more severe deficits in neuronal differentiation and projection in many other brain regions. These results indicate that Fezf2 may function differently in the mammillary body. Our results provide important insights for mammillary development and connectivity. |
