| First Author | Lu J | Year | 2019 |
| Journal | Neuroscience | Volume | 422 |
| Pages | 146-160 | PubMed ID | 31678344 |
| Mgi Jnum | J:285587 | Mgi Id | MGI:6391877 |
| Doi | 10.1016/j.neuroscience.2019.09.027 | Citation | Lu J, et al. (2019) Increased Type I and Decreased Type II Hair Cells after Deletion of Sox2 in the Developing Mouse Utricle. Neuroscience 422:146-160 |
| abstractText | The vestibular system of the inner ear contains Type I and Type II hair cells (HCs) generated from sensory progenitor cells; however, little is known about how the HC subtypes are formed. Sox2 (encoding SRY-box 2) is expressed in Type II, but not in Type I, HCs. The present study aimed to investigate the role of SOX2 in cell fate determination in Type I vs. Type II HCs. First, we confirmed that Type I HCs developed from Sox2-expressing cells through lineage tracing of Sox2-positive cells using a CAG-tdTomato reporter mouse crossed with a Sox2-CreER mouse. Then, Sox2 loss of function was induced in HCs, using Sox2(flox) transgenic mice crossed with a Gfi1-Cre driver mouse. Knockout of Sox2 in HCs increased the number of Type I HCs and decreased the number of Type II HCs, while the total number of HCs and Sox2-positive supporting cells did not change. In addition, the effect of Sox2-knockout persisted into adulthood, resulting in an increased number of Type I HCs. These results demonstrate that SOX2 plays a critical role in the determination of Type II vs. Type I HC fate. The results suggested that Sox2 is a potential target for generating Type I HCs, which may be important for regenerative strategies for balance disorders. |
