| Primary Identifier | MGI:3808779 | Allele Type | Targeted |
| Gene | Fen1 | Transmission | Germline |
| Strain of Origin | 129X1/SvJ | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Site directed mutagenesis followed by homologous recombination was used to modify the coding sequence of exon 2 creating an amino acid substitution of aspartic acid for glutamic acid at position 160 (E160D). This mutation alters the single active residue site in the nuclease core domain. A neo cassette was also inserted downstream of exon 2 for selection purposes. |
