| Primary Identifier | MGI:3808783 | Allele Type | Targeted |
| Gene | Fen1 | Transmission | Germline |
| Strain of Origin | 129X1/SvJ | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Site directed mutagenesis followed by homologous recombination was used to modify the coding sequence of exon 2 creating amino acid substitutions at positions 343 and 344 of glycine and alanine, respectively, for phenylalanine (F343G and F344A). This mutation alters two amino acids critical for protein protein interaction. A neo cassette was also inserted downstream of exon 2 for selection purposes. |
