| First Author | Ernesto JI | Year | 2015 |
| Journal | J Cell Biol | Volume | 210 |
| Issue | 7 | Pages | 1213-24 |
| PubMed ID | 26416967 | Mgi Jnum | J:227650 |
| Mgi Id | MGI:5702363 | Doi | 10.1083/jcb.201412041 |
| Citation | Ernesto JI, et al. (2015) CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization. J Cell Biol 210(7):1213-24 |
| abstractText | Ca(2+)-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca(2+) channels. Specifically, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilization of cumulus-oocyte complexes from CRISP1 knockout females is impaired because of a failure of sperm to penetrate the cumulus. We provide evidence that CRISP1 stimulates sperm orientation by modulating sperm hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, patch clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca(2+) channel involved in hyperactivation and essential for fertility. Given the critical role of Ca(2+) for sperm motility, we propose a novel CRISP1-mediated fine-tuning mechanism to regulate sperm hyperactivation and orientation for successful penetration of the cumulus during fertilization. |
