Primary Identifier | MGI:3809654 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Cd83 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A reporter cassette consisting of eGFP gene linked to a viral IRES2 sequence was positioned right after the stop codon located in exon 5 of the gene. A neomycin resistance gene was included after the EGFP stop codon. The entire coding region of the gene remained intact in the targeting construct. Expression of the targeted gene generated a bicistronic transcript encoding both CD83 and eGFP. |