| Primary Identifier | MGI:3811006 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Constitutively active, Inserted expressed sequence, Reporter | Gene | Tg(CAG-lacZ,-Akt1*,-EGFP)56Ebm |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This transgene consists of a chicken beta-actin promoter with the upstream cytomegalovirus enhancer (pCAGGS) followed by a floxed lacZ neo fusion cassette with three SV40 poladenylation sites, a constitutively active form of Akt1 with an HA epitope, an IRES site, and enhanced GFP. Cre-mediated recombination allows for expression of the constitutively active Akt1 and EGFP. Two lines were generated (42 and 56) that both had strong homogeneous lacZ expression. This is a representative record for these two lines. |
