| Primary Identifier | MGI:3811233 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Pkd1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP-flanked neomycin selection cassette was inserted into the EcoRI site located 1.2 kb upstream of exon 2, and a third loxP site placed downstream of exon 6. Chimeric mice were crossed with Tg(Prm-cre)58Og transgenic mice that express cre recombinase in male germ cells. Offspring were selected that had the neomycin cassette excised, leaving exons 2 through 6 flanked by loxP sites. Correct targeting and excision was confirmed by Southern blotting. |
