| Primary Identifier | MGI:3812034 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Itsn1 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neo cassette with a 5' loxP site was inserted upstream of exon 3 and an additional loxP site was inserted downstream of exon 3. Transient cre expression in ES cells was used to removed the neo cassette and exon 3 causing a frame shift and premature termination of translation. The absence of the short and long forms of the protein was confirmed by western blot analysis on brain and heart extracts. |
