| First Author | Ye Y | Year | 2014 |
| Journal | Cell Signal | Volume | 26 |
| Issue | 11 | Pages | 2514-20 |
| PubMed ID | 25093805 | Mgi Jnum | J:264665 |
| Mgi Id | MGI:6192970 | Doi | 10.1016/j.cellsig.2014.07.030 |
| Citation | Ye Y, et al. (2014) Ablation of the GNB3 gene in mice does not affect body weight, metabolism or blood pressure, but causes bradycardia. Cell Signal 26(11):2514-20 |
| abstractText | G protein beta3 (Gbeta3) is an isoform of heterotrimeric G protein beta subunits involved in transducing G protein coupled receptor (GPCR) signaling. Polymorphisms in Gbeta3 (GNB3) are associated with many human disorders (e.g. hypertension, diabetes and obesity) but the role of GNB3 in these pathogeneses remains unclear. Here, Gbeta3-null mice (GNB3(-/-)) were characterized to determine how Gbeta3 functions to regulate blood pressure, body weight and metabolism. We found Gbeta3 expression restricted to limited types of tissues, including the retina, several regions of the brain and heart ventricles. Gbeta3-deficient mice were normal as judged by body weight gain by age or by feeding with high-fat diet (HFD); glucose tolerance and insulin sensitivity; baseline blood pressure and angiotensin II infusion-induced hypertension. During tail-cuff blood pressure measurements, however, Gbeta3-null mice had slower heart rates (~450 vs ~500 beats/min). This bradycardia was not observed in isolated and perfused Gbeta3-null mouse hearts. Moreover, mouse hearts isolated from GNB3(-/-) and controls responded equivalently to muscarinic receptor- and beta-adrenergic receptor-stimulated bradycardia and tachycardia, respectively. Since no difference was seen in isolated hearts, Gbeta3 is unlikely to be involved directly in the GPCR signaling activity that controls heart pacemaker activity. These results demonstrate that although Gbeta3 appears dispensable in mice for the regulation of blood pressure, body weight and metabolic features associated with obesity and diabetes, Gbeta3 may regulate heart rate. |
