| First Author | Hara T | Year | 2019 |
| Journal | Cancer Res | Volume | 79 |
| Issue | 15 | Pages | 3983-3991 |
| PubMed ID | 31315836 | Mgi Jnum | J:278165 |
| Mgi Id | MGI:6342557 | Doi | 10.1158/0008-5472.CAN-19-0717 |
| Citation | Hara T, et al. (2019) Modeling Gliomas Using Two Recombinases. Cancer Res 79(15):3983-3991 |
| abstractText | Development of animal models to investigate the complex ecosystem of malignant gliomas using the Cre/loxP recombination system has significantly contributed to our understanding of the molecular underpinnings of this deadly disease. In these model systems, once the tumor is induced by activation of Cre-recombinase in a tissue-specific manner, further genetic manipulations to explore the progression of tumorigenesis are limited. To expand the application of mouse models for gliomas, we developed glial fibrillary acidic protein (GFAP)-FLP recombinase (FLPo) mice that express FLPo recombinase specifically in GFAP-positive cells. Lentivirus-based in vivo delivery of cancer genes conditioned by FLP/FRT-mediated recombination initiated gliomas in GFAP-FLPo mice. Using the Cre-mediated multifluorescent protein-expressing system, we demonstrated that the GFAP-FLPo mouse model enables the analysis of various stages of gliomagenesis. Collectively, we present a new mouse model that will expand our ability to dissect developmental processes of gliomagenesis and to provide new avenues for therapeutic approaches. SIGNIFICANCE: This study presents a new glioma mouse model derived using lentiviral vectors and two recombination systems that will expand the ability to dissect developmental processes of gliomagenesis. |
