Primary Identifier | MGI:3814427 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Trpa1 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | LoxP sites were placed on either side of exons 22-24 (encoding the S5/S6 transmembrane domains) and an FRT-flanked neomycin resistance cassette inserted in the opposite transcriptional orientation in intron 24. The neomycin cassette was excised by crossing these mice to a Gt(ROSA)26Sor-driven Flp strain on a 129S4/SvJaeSor. |