| Primary Identifier | MGI:3817518 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Zbtb7b |
| Transmission | Germline | Strain of Origin | (129 x C57BL/6)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | Mutant ES cell line = M1 heterozygous for the Zbtb7btm1Tani allele |
| molecularNote | ES cells containing the GFP knock-in allele, Zbtb7btm1Tani, were targeted for a second mutation. A loxP site was placed approximately 6 kb upstream of the transcription start site, placing it 5' to a proximal enhancer for this gene locus. A floxed neo-cassette was placed downstream of the enhancer. Transient expression of cre-recombinase removed a 2.2-kb region including the proximal enhancer and the neo cassette. Southern blotting confirmed correct targeting of the knock in allele. |
