| Primary Identifier | MGI:3820285 | Allele Type | Targeted |
| Gene | Tgfb1 | Transmission | Germline |
| Strain of Origin | 129/SvEv | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Cysteine codon 33 (TGC) was changed to serine (TCC) (p.C33S). A loxP site flanked neomycin resistance gene cassette was included in the targeting vector and was subsequently removed by crossing founder mice with cre transgenic mice. The mutated amino acid is located in the latency-associated peptide portion of the precursor protein that becomes non-covalently associated with the mature but biologically inactive (latent) form of TGF-beta. This mutation disrupts the association of the latency-associated peptide to latent TGF-beta binding proteins. Latent TGF-beta from the sera or platelets of homozygotes is not activated by shear forces. |
