| Primary Identifier | MGI:3821721 | Allele Type | Targeted |
| Gene | H2-T23 | Transmission | Germline |
| Strain of Origin | 129S6/SvEvTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exon 4 of the gene locus was targeted with a mutation that causes an aspartic acid to lysine exchange at amino acid position 227 (D227K). A floxed neomycin resistance cassette was also inserted downstream of exon 5 and was subsequently removed by crossing chimeric mice with a cre transgenic strain. The mutant protein was expressed on the surface of activated CD4+ T cells from homozygous mice at levels similar to the endogenous protein in wild-type mice. This mutation was predicted to disrupt binding to the CD8 coreceptor complex. |
