| Primary Identifier | MGI:3822473 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Hesx1 |
| Transmission | Germline | Strain of Origin | 129S/SvEv-Gpi1<c> |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The coding region of the Hesx1 gene was replaced with sequence encoding Cre recombinase, a polyA sequence and an Frt-flanked PGK-Neo cassette, via homologous recombination. The targeting vector was electroporated into CCE ES cells. ES cells were injected into B6 blastocysts to generate mice. Subsequently, the Pgk-Neo cassette was excised by crossing mice to ACTB:FLPe transgenic animals and maintained on a C57BL/6J background. |
