| Primary Identifier | MGI:3821884 | Allele Type | Transgenic |
| Attribute String | Conditional ready, Constitutively active, Humanized sequence, Inserted expressed sequence, Reporter | Gene | Tg(CAG-LacZ,-ACVR1*,-EGFP)35-1Mis |
| Strain of Origin | (C57BL/6 x DBA/2)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | Phenotypic Similarity to Human Syndrome: Submucous cleft palate J:233662 in mice hemizygous for Tg(CAG-LacZ,-ACVR1*,-EGFP)35-1Mis and Tg(KRT14-cre)1Amc. |
| molecularNote | The modified chicken beta-actin promoter (CAG) was used to drive ubiquitous expression of a floxed LacZ gene with a triple polyadenylation signal sequence followed by a constitutively active human ACVR1 sequence with the amino acid substitution of glutamine with aspartic acid at position 207 (Q207D), an IRES sequence, and the EGFP coding sequence and a poly(A) signal. Five lines were established and expression analysis for one line (line 35-1) confirmed general, but not ubiquitous, expression of LacZ and cre-dependent general expression of the modified ACVR1 and EGFP. |
