Primary Identifier | MGI:3828307 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Adar |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed neo selection cassette was placed upstream of exon 7 in the SmaI restriction site and an additional loxP site was inserted downstream of exon 9. Correct targeting was confirmed by genomic PCR. LoxP-flanked (floxed) sequence encoded residues 707?870 of the interferon-inducible form of ADAR1 (GenBankTM/EBI accession number AAK16102), including essential regions for editing activity. |