| First Author | Zhao J | Year | 2010 |
| Journal | Mol Cell | Volume | 40 |
| Issue | 6 | Pages | 939-53 |
| PubMed ID | 21172659 | Mgi Jnum | J:168094 |
| Mgi Id | MGI:4881870 | Doi | 10.1016/j.molcel.2010.12.011 |
| Citation | Zhao J, et al. (2010) Genome-wide identification of polycomb-associated RNAs by RIP-seq. Mol Cell 40(6):939-53 |
| abstractText | Polycomb proteins play essential roles in stem cell renewal and human disease. Recent studies of HOX genes and X inactivation have provided evidence for RNA cofactors in Polycomb repressive complex 2 (PRC2). Here we develop a RIP-seq method to capture the PRC2 transcriptome and identify a genome-wide pool of >9000 PRC2-interacting RNAs in embryonic stem cells. The transcriptome includes antisense, intergenic, and promoter-associated transcripts, as well as many unannotated RNAs. A large number of transcripts occur within imprinted regions, oncogene and tumor suppressor loci, and stem cell-related bivalent domains. We provide evidence for direct RNA-protein interactions, most likely via the Ezh2 subunit. We also identify Gtl2 RNA as a PRC2 cofactor that directs PRC2 to the reciprocally imprinted Dlk1 coding gene. Thus, Polycomb proteins interact with a genome-wide family of RNAs, some of which may be used as biomarkers and therapeutic targets for human disease. |
