| Primary Identifier | MGI:3830280 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Il17f |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A reporter construct was made consisting of an IRES domain, coding sequence for a variant of murine Thy1 (Thy1.1), and a loxP-flanked neomycin cassette. The vector was targeted to exon 3 of the gene locus immediately downstream of the stop codon and within the 3' untranslated portion of the gene. Chimeric founders were bred with B6.EIIa-Cre transgenic mice to remove the neomycin selection cassette. CD4+ T cells isolated from homozygote mice and cultured under Th17 conditions co-expressed both IL-17F and Thy1.1 in a log-linear manner. |
