| Primary Identifier | MGI:3831153 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Traf3ip2 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 3.6 Kb BamHI fragment containing exon 2 was replaced with a neomycin selection cassette. Correct targeting was confirmed by Southern blotting. Analysis of mRNAs from homozygote B cells demonstrates exon 1 was spliced to exon 3. All potential ATG start codons upstream of exon 3 and located in the correct reading frame are followed by at least 2 stop codons prior to the start of exon 3. The first ATG present within the functional reading frame downstream of the start of exon 3 is located in exon 5, which could only generate a 157 amino acid long fragment of the 555 amino acid endogenous protein. For these reasons, a functional protein product was considered unlikely. |
