| Primary Identifier | MGI:3829597 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Sohlh2 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:143535 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
| molecularNote | Exons 2 through 8 were replaced with an IRES beta-geo cassette and puromycin selection marker. The absence of protein expression was confirmed by western blot analysis on testes extracts. |
