Primary Identifier | MGI:3830362 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Inpp5d |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 1 and a floxed neomycin cassette was placed downstream of exon 1. Cre recombinase was transiently expressed in correctly targeted ES cells to remove the neomycin selection cassette, leaving exon 1 flanked by loxP sites. Genomic PCR and Southern blotting confirmed removal of the neo cassette and the flox-state of exon 1. |