Primary Identifier | MGI:3831084 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | En2 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct contains a 3.1 kb En2 5' fragment, an Frt-flanked PGK-neo cassette followed by a LoxP site in the En2 intron and a 4.8 kb En2 genomic fragment containing a LoxP site in the XbaI site of the 3' UTR. Flpe-mediated recombination deleted the PGK-neo, producing an En2 allele with a floxed region. |