| Primary Identifier | MGI:3833549 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A selection marker and a transcriptional stop cassette were flanked by loxP sites and placed upstream of a chimeric gene. This chimeric gene encodes the transactivation domains of Tcfe2a (E47) and the bHLH domain of Tal1 (SCL). An IRES-GFP cassette was placed downstream of the chimeric gene and the whole construct (loxP-neo-STOP-loxp- Tcfe2a/Tal1-IRES-GFP) was knocked into the locus. Upon expression of cre recombinase, the chimeric protein and GFP will be expressed, with the chimeric protein predicted to have a dominant negative effect on members of the Id and Tal protein family due to its strong affinity for E transcription factors. |
