| Primary Identifier | MGI:3836312 | Allele Type | Targeted |
| Gene | Igh | Transmission | Germline |
| Strain of Origin | B6(Cg)-Tyr<c-2J> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A targeting vector containing a self-excising loxP-flanked neomycin/sperm-specific Cre cassette was used to target a loxP site and a I-SceI endonuclease site upstream of the S-mu switching region and downstream of the E-mu enhancer. The construct was electroporated into albino C57BL/6 embryonic stem (ES) cells. Correctly targeted ES clones were injected into recipient blastocysts. The resulting male founder had self-excision of the neomycin/Cre cassette, leaving the loxP and I-SceI sites in the germline. Correct targeting was confirmed by Southern blotting. B cell development and numbers were normal in heterozygote mice. |
