| Primary Identifier | MGI:3835520 | Allele Type | Targeted |
| Attribute String | Recombinase, Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | true | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| description | Germ line transmission of mutant cell line mEMS756 has been established. |
| molecularNote | The Ple178-EGFPCre transgene (pEMS1090) was designed with the 864 bp Ple178 minipromoter (RGS16-D; derived from an intronic candidate regulatory region and a subsection of the human regulator of G-protein signaling 16 (RGS16) promoter) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). |
