| Primary Identifier | MGI:3835522 | Allele Type | Targeted |
| Attribute String | Recombinase, Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | true | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| description | Germ line transmission of mutant cell line mEMS759 has been established. |
| molecularNote | The Ple176-EGFPCre transgene (pEMS1088) was designed with the 3231 bp Ple176 minipromoter (RGS16-B; derived from two upstream candidate regulatory regions and a subsection of the human regulator of G-protein signaling 16 (/RGS16/) promoter) upstream of a minimal F5 mutant-/frt/ site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal /frt/ site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). |
