| Primary Identifier | MGI:3835525 | Allele Type | Targeted |
| Attribute String | Recombinase, Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | true | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| description | Germ line transmission of mutant cell line mEMS675 has been established. |
| molecularNote | The Ple103-EGFP/cre transgene (pEMS1083) was designed with the 877 bp Ple103 minipromoter (HAP1-A; derived from a segment of the basal promoter element of the human huntingtin-associated protein 1 (HAP1) gene) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtsb-m3 mutant locus on the X chromosome. |
