| Primary Identifier | MGI:3835534 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| description | Germ line transmission of mutant cell line mEMS1892 has been established. |
| molecularNote | The Ple53-EGFP transgene (pEMS1199) was designed with the 3520 bp Ple53 minipromoter (DCX-B; derived from a portion of the promoter of the DCX gene from the doublecortin (DCX) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. EGFP-immunoreactivity is detected most strongly in the neurogenic cells of the rostral migratory stream (RMS). |
