| Primary Identifier | MGI:3835992 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tmpo |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | At least six alternative transcripts can be derived from this gene locus. This mutation targeted exon 4, which is necessary for generation of a protein product that localizes to the nucleoplasm. A loxP site and a FRT-flanked neomycin cassette was placed upstream of exon 4 and an additional loxP site was placed downstream of the exon. Founder mice were crossed with Cre-deleter mice to remove exon 4 and the neomycin cassette. Homozygote mice lacked the nucleoplasm specific protein isoform but expressed the other protein isoforms as determined by immunoblot analysis of heart and intestinal extracts. |
