| First Author | Grimm C | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 4 | Pages | 101761 |
| PubMed ID | 36240060 | Mgi Jnum | J:353125 |
| Mgi Id | MGI:7663156 | Doi | 10.1016/j.xpro.2022.101761 |
| Citation | Grimm C, et al. (2022) An optimized protocol for assessing changes in mouse whole-brain activity using opto-fMRI. STAR Protoc 3(4):101761 |
| abstractText | Functional magnetic resonance imaging (fMRI) in mouse brain, paired with spatially and temporally defined manipulations, offers a powerful tool to causally explain the effect of specific neuronal activity on brain network dynamics. Here, we present an optimized protocol to measure cell-type-specific contributions to changes in whole-brain dynamics in mice using optogenetics (opto)-fMRI. This protocol details the injection of ChR2-expressing AAV, the implantation of optical fiber, the steps to perform opto-BOLD (blood-oxygenation-level-dependent) fMRI recording, and data analysis. For complete details on the use and execution of this protocol, please refer to Grimm et al. (2021). |
