| First Author | Broussard GJ | Year | 2018 |
| Journal | Nat Neurosci | Volume | 21 |
| Issue | 9 | Pages | 1272-1280 |
| PubMed ID | 30127424 | Mgi Jnum | J:273439 |
| Mgi Id | MGI:6257267 | Doi | 10.1038/s41593-018-0211-4 |
| Citation | Broussard GJ, et al. (2018) In vivo measurement of afferent activity with axon-specific calcium imaging. Nat Neurosci 21(9):1272-1280 |
| abstractText | In vivo calcium imaging from axons provides direct interrogation of afferent neural activity, informing the neural representations that a local circuit receives. Unlike in somata and dendrites, axonal recording of neural activity-both electrically and optically-has been difficult to achieve, thus preventing comprehensive understanding of neuronal circuit function. Here we developed an active transportation strategy to enrich GCaMP6, a genetically encoded calcium indicator, uniformly in axons with sufficient brightness, signal-to-noise ratio, and photostability to allow robust, structure-specific imaging of presynaptic activity in awake mice. Axon-targeted GCaMP6 enables frame-to-frame correlation for motion correction in axons and permits subcellular-resolution recording of axonal activity in previously inaccessible deep-brain areas. We used axon-targeted GCaMP6 to record layer-specific local afferents without contamination from somata or from intermingled dendrites in the cortex. We expect that axon-targeted GCaMP6 will facilitate new applications in investigating afferent signals relayed by genetically defined neuronal populations within and across specific brain regions. |
