| First Author | Augustinaite S | Year | 2020 |
| Journal | STAR Protoc | Volume | 1 |
| Issue | 3 | Pages | 100194 |
| PubMed ID | 33377088 | Mgi Jnum | J:349553 |
| Mgi Id | MGI:6727728 | Doi | 10.1016/j.xpro.2020.100194 |
| Citation | Augustinaite S, et al. (2020) Chronic Cranial Window for Imaging Cortical Activity in Head-Fixed Mice. STAR Protoc 1(3):100194 |
| abstractText | Chronic cranial window surgery is a critical procedure for in vivo imaging in neuroscience. Here, we describe our surgical protocol with several subtle improvements that increase the success rate significantly. The window allows high-quality imaging in head-fixed behaving mice within the first week after the surgical procedure and remains clear for months. We used this procedure to prepare mice for intrinsic signal imaging and two-photon imaging of layer 6 neurons in visual cortex. For complete details on the use and execution of this protocol, please refer to Augustinaite and Kuhn (2020). |
