| Primary Identifier | MGI:3837565 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Cert1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene trap vector, pGT2Lxf, was inserted between exons 3 and 4 disrupting the pleckstrin homology domain and resulting in a truncated protein product. The exact site of insertion was 18394 nucleotides downstream of the 5' end of the third intron. RT-PCR analysis failed to detect full length transcript in homozygous embryos. Western blot analysis detected little or no protein expression in homozygous embryos. |
