Primary Identifier | MGI:3838373 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Usp1 |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 3 was flanked by loxP sites with a FRT-flanked neomycin resistance cassette placed downstream. Before microinjection into blastocystes, the neomycin cassette was excised by transient expression of Flp-recombinase. Mice were crossed with EIIa-cre transgenic mice to remove the floxed allele. No protein was detected by immunoblot analysis of homozygote embryonic fibroblast lysates. |