Primary Identifier | MGI:3837686 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ccm2 |
Transmission | Germline | Strain of Origin | 129 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed GFP/hygromycin resistance cassette was placed upstream of exon 3 and an additional loxP site was placed downstream of exon 4. Chimeric mice were bred with Tg(rtetR-TetO-cre)40Mhz mice to remove the selection cassette and exons 3 and 4. Gene inactivation was confirmed by a lack of protein detected in immunoblot analysis of E9.5 embryo lysates. |