| Primary Identifier | MGI:3838510 | Allele Type | Targeted |
| Attribute String | Conditional ready, Constitutively active, Humanized sequence | Gene | Pdgfra |
| Transmission | Germline | Strain of Origin | 129S4/SvJaeSor |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 2 through 4 were replaced with a cDNA of the gene with nucleotide substitutions that result in an amino acid substitution of aspartic acid for valine at position 561 (V561D) preceded by a floxed neo-STOP cassette. This mutation is found in human patients with gastrointestinal stromal tumors and leads to a constitutively active form by disrupting the inhibitory conotacts between the juxtamembrane and kinase domain. Removal of the floxed neo-STOP cassette is necessary to achieve expression. |
