| First Author | Kondoh G | Year | 1999 |
| Journal | FEBS Lett | Volume | 458 |
| Issue | 3 | Pages | 299-303 |
| PubMed ID | 10570928 | Mgi Jnum | J:106691 |
| Mgi Id | MGI:3619264 | Doi | 10.1016/s0014-5793(99)01172-2 |
| Citation | Kondoh G, et al. (1999) Tissue-inherent fate of GPI revealed by GPI-anchored GFP transgenesis. FEBS Lett 458(3):299-303 |
| abstractText | To clarify the fate of glycosylphosphatidylinositol (GPI) in mammals, we developed GPI-anchored enhanced green fluorescent protein (EGFP-GPI) and transgenic mice carrying this fusion construct. When it was introduced to culture cells, the EGFP-GPI protein was correctly sorted to plasma membranes and microsomes depending on GPI biosynthesis. Transgenic mice carrying EGFP-GPI were found to show a broad transgene expression. Histologically, a prominent polarized localization of EGFP-GPI protein was observed in various epithelia, the nervous system and liver and secreted from some exocrine glands, as well as non-polarized presence in non-epithelial tissues, demonstrating a tissue-inherent manner of GPI sorting. |
