Primary Identifier | MGI:3840213 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6J)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A vector containing a CAG promoter driving expression of a floxed neo/STOP cassette, frt flanked lacZ gene with 3 polyadenylation sequences (secondary STOP cassette), and an EGFP gene was inserted into the locus. Germ line, flp mediated recombination removes the lacZ/STOP cassette leaving the neo/STOP cassette intact. Cre mediated recombination is necessary to achieve EGFP expression. |