| Primary Identifier | MGI:3840617 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Cdr2 |
| Transmission | Germline | Strain of Origin | 129P2/Ola |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A vector was designed to replace the majority of the targeted gene's coding region - exon 2, all of exons 3 and 4, as well as the coding region in exon 5. EGFP was fused in-frame to the coding sequence within exon 2, followed by an FRT site, 1.3kb of the human glyceraldehyde-3-phosphate dehydrogenase (GAPD) 3'UTR and flanking sequence, a loxP-flanked neomycin cassette, and a second FRT site. The construct was electroporated into 129P2/Ola-derived embryonic stem (ES) cells. Correctly targeted ES cells were transfected with a cre-expressing plasmid to remove the neomycin cassette. |
