Other
12 Authors
- Zhou L,
- Zhou M,
- McPherson L,
- Clayberger C,
- Wang D,
- Song A,
- Lyu SC,
- Shi X,
- Krensky AM,
- Ahn YT,
- Dong C,
- Feng D
| First Author | Zhou M | Year | 2007 |
| Journal | J Immunol | Volume | 178 |
| Issue | 9 | Pages | 5496-504 |
| PubMed ID | 17442931 | Mgi Jnum | J:145839 |
| Mgi Id | MGI:3836134 | Doi | 10.4049/jimmunol.178.9.5496 |
| Citation | Zhou M, et al. (2007) Kruppel-like transcription factor 13 regulates T lymphocyte survival in vivo. J Immunol 178(9):5496-504 |
| abstractText | Kruppel-like transcription factor (KLF)13, previously shown to regulate RANTES expression in vitro, is a member of the Kruppel- like family of transcription factors that controls many growth and developmental processes. To ascertain the function of KLF13 in vivo, Klf13-deficient mice were generated by gene targeting. As expected, activated T lymphocytes from Klf13(-/-) mice show decreased RANTES expression. However, these mice also exhibit enlarged thymi and spleens. TUNEL, as well as spontaneous and activation-induced death assays, demonstrated that prolonged survival of Klf13(-/-) thymocytes was due to decreased apoptosis. Microarray analysis suggests that protection from apoptosis-inducing stimuli in Klf13(-/-) thymocytes is due in part to increased expression of BCL-X(L), a potent antiapoptotic factor. This finding was confirmed in splenocytes and total thymocytes by real-time quantitative PCR and Western blot as well as in CD4+CD8- single-positive thymocytes by real-time quantitative PCR. Furthermore, EMSA and luciferase reporter assays demonstrated that KLF13 binds to multiple sites within the Bcl-X(L) promoter and results in decreased Bcl-X(L) promoter activity, making KLF13 a negative regulator of BCL-X(L). |
