| Primary Identifier | MGI:3842838 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Vpreb1b |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cells heterozygous for Vpreb1tm1Ilm were electroporated with the targeting vector. Three targeted mutant lines identified as ES47, ES65, and ES223 were found in which targeted insertion occurred trans to the pre-existing mutant allele. |
| molecularNote | A single copy of a construct in which the entire coding region was replaced with a hygromycin cassette was incorporated at the endogenous locus via homologous recombination. Insertion occurred trans to the pre-existing mutant allele. Transcript was undetected by RT-PCR analysis of bone marrow cells obtained from homozygous mutant mice. The absence of protein surface expression was confirmed by FACS analysis. |
