| Primary Identifier | MGI:3844631 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cpn1 |
| Transmission | Germline | Strain of Origin | 129S1/SvImJ |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EZ-1 |
| molecularNote | Exon 3 of the locus was replaced by a neomycin resistance cassette. RT-PCR analysis on homozygous liver extracts detected the presence of a truncated mRNA message corresponding to a splice variant that fused exon 2 to exon 4. Because exon 3 encoded a substrate binding site, it was hypothesized that any mutant protein transcribed from the truncated mRNA would lack carboxypeptidase activity. This was confirmed by a lack of terminal arginine cleavage on C3a and C5a complement proteins. |
