| Primary Identifier | MGI:3845022 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Hras |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| description | Phenotypic Similarity to Human Syndrome: Thyroid Cancer J:231492 in conditional mice homozygous for Hrastm1Jaf and homozygous for either Nf2tm2Gth, Trp53tm1Brn or Ptentm2.1Ppp. |
| molecularNote | The targeted locus consisted of 2 tandemly-arrayed murine Hras1 genes with the upstream wild-type copy flanked by loxP sites and the downstream copy containing a glycine-to-valine mutation at amino acid 12 (G12V). Between the two genes was placed a SV40 poly A sequence and a FRT-flanked neomycin selection cassette that was subsequently removed by crossing with transgenic mice expressing the FLPe recombinase. In the absence of cre expression, only the wild-type gene is expressed. In the presence of cre recombinase, the wild-type gene is excised and the mutant gene is instead expressed. |
