Primary Identifier | MGI:3846573 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Fat4 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
description | ES cell line = 129/1, a subline of GK129. |
molecularNote | A loxP site was inserted upstream of exon 3 and a loxP site and frt flanked PGK-neo cassette were inserted downstream of exon 3 via homologous recombination. Two mutant ES cell lines acquired the two loxP insertion in the targeted gene. This allele is derived from line 2E11 in which targeting occured as expected. Subsequent Cre mediated recombination removed exon 3. |