| First Author | Tang JC | Year | 2015 |
| Journal | Nat Neurosci | Volume | 18 |
| Issue | 9 | Pages | 1334-41 |
| PubMed ID | 26258682 | Mgi Jnum | J:225871 |
| Mgi Id | MGI:5694858 | Doi | 10.1038/nn.4081 |
| Citation | Tang JC, et al. (2015) Cell type-specific manipulation with GFP-dependent Cre recombinase. Nat Neurosci 18(9):1334-41 |
| abstractText | There are many transgenic GFP reporter lines that allow the visualization of specific populations of cells. Using such lines for functional studies requires a method that transforms GFP into a molecule that enables genetic manipulation. We developed a method that exploits GFP for gene manipulation, Cre recombinase dependent on GFP (CRE-DOG), a split component system that uses GFP and its derivatives to directly induce Cre/loxP recombination. Using plasmid electroporation and AAV viral vectors, we delivered CRE-DOG to multiple GFP mouse lines, which led to effective recombination selectively in GFP-labeled cells. Furthermore, CRE-DOG enabled optogenetic control of these neurons. Beyond providing a new set of tools for manipulation of gene expression selectively in GFP(+) cells, we found that GFP can be used to reconstitute the activity of a protein not known to have a modular structure, suggesting that this strategy might be applicable to a wide range of proteins. |
