| Primary Identifier | MGI:3847088 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Stk4 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted into a Ssp I restriction site 1.1 kb upstream of exon 1, and a floxed neomycin cassette was placed 1 kb downstream of exon 1. ES cells with the targeted locus were transiently infected with a cre-expressing adenovirus to generate exon 1 flanked with loxP sites. Expression of the locus was not affected as determined by immunoblot analysis of spleen, thymus, brain and lung tissues. |
