| Primary Identifier | MGI:3848053 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Glis3 |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 4, which contains the sequence coding for zinc finger 5, and parts of intron 3 and 4 were replaced with a neo cassette. Deletion of zinc finger 5 (ZF5) is predicted to disrupt DNA binding ablity and transcriptional activity of the gene. The inability of the protein produced from this allele to activate transcription of a luciferase reporter with a Glis-binding site was confirmed by cellular assay. |
